Class 1 Laser Product. Compact and easy to use, the BD Accuri is a highly sensitive tool, providing the investigator with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function. The ability to perform hands-on research using BD Accuri systems helps these young scientists understand what it's like to work in a modern research lab. Data courtesy of Gary Wikfors, PhD, NOAA, Milford, CT. A 1-L bioreactor was batched with 900 mL of medium and inoculated with 100 mL of spinner flask culture of Chinese hamster ovary (CHO) cells at 106 cells/mL. In addition to apoptosis, cell cycle, and proliferation studies, immunophenotyping is essential for understanding the cellular ecology of cancer cells. No. BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Solutions to Enable Your COVID-19 Research, BD Horizon™ Guided Panel Solution (GPS) tool. Alternatively, you can select User Defined Compensation Settings to calculate compensation manually, which is also necessary for other fluorochromes or dyes. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Free BD Accuri software templates simplify data collection and analysis. Standard fluorochrome in use are identical to FACS Calibur. Multicolor flow cytometry is ideal for this purpose because of its ability to interrogate heterogeneous cell populations, analyze their subpopulations, and characterize multiple antigens simultaneously. *Install the BD Accuri C6 Software. Training can be requested via PPMS. Figure 7 Figure 8 Figure 9 7. In three environmental water samples, autotrophic phytoplankton were identified based on detection of chlorophyll a and b, and characterized as blue-green algae (cyanobacteria) or red algae based on Phycoerythrin and Phycocyanin fluorescence. 564696, upper plots) or MitoStatus Red (Cat. 561527), stained according to kit instructions, and acquired on a BD Accuri C6 flow cytometer using the kit template. In the diverse field of microbiology research, flow cytometry is a powerful technique for analyzing microorganisms including bacteria and yeast, and offers many advantages over more conventional techniques. This protocol also assumes a completed assay in a 96-well plate, with particles suspended in Run buffer. You can add additional reagents to screen cell populations, monitor DNA content, identify cells at different phases of the cell cycle, and examine heterogeneity and transient effects in reporter expression. Comparative cytograms of Fluo-4 fluorescence of C6 glioma cells over time, showing the effects of adding control and test compounds (ionophore A23187, ethanol, and thapsigargin). Access to training videos presented by FCR staff are here. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. Free BD Accuri software templates simplify data collection and analysis. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. incorporates efficient collection optics and two lasers(488-blue and 633 red) to analyze up to four colors. Like ELISA assays, BD™ Cytometric Bead Array (CBA) assays measure secreted proteins produced by entire cell populations. BD Accuri systems can measure the expression of Green, Yellow, or Red Fluorescent Protein (GFP, YFP, or RFP), as well as other fluorescent proteins, in their standard configurations. Flow cytometry offers a powerful and effective method for screening microalgal cultures, and BD Accuri systems are especially well suited to the task. BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all … Continuous, gap-free recording of intracellular calcium levels on the BD Accuri C6, Interaction of nanoparticles with mammalian cells, Continuous sampling at varying temperatures. The fully digital BD Accuri C6 features two lasers, two scatter detectors, and four fluorescence detectors. They were acquired on a BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software, gating on lymphocytes using light scatter and then on CD3+CD8+ cytotoxic T cells. Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. Rapid and accurate quantitation of bacteria in drinking water is essential to monitor, control, and optimize treatment processes and to illuminate the biology of low-nutrient water systems. Students perform cell staining with an antibody panel designed to differentiate various white blood cell (WBC) populations (see figure). Samples containing wild-type (left column) or GFP-transfected (middle column). Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a significant research challenge. All tasks and settings are laid out simply on one screen for quick access and operation. 560484), acquired on a BD Accuri C6 using the BD™ CBA Kit Accuri template, and analyzed using FCAP Array software v3.0.1. BD Accuri™ C6 BD FACSCalibur™ BD FACSVerse™* BD FACSCanto™ II BD LSRFortessa™ X-20 BD FACSAria™ Product Family BD Influx™ BD FACSJazz™ Excitation Laser Line Filter Relative Brightness Blue Laser (488 nm) / Yellow-Green Laser (561 nm) •••••••• 488 nm … The direct counts correlate highly with counting beads (r. Using the BD Pharmingen Human Th1/Th2/Th17 Phenotyping Kit (Cat. Direct observation and experimentation are widely becoming central to a strong undergraduate education in biology as well as other disciplines. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends. The BD Accuri C6 Plus can examine post-translational modifications of cell signaling proteins. Optional filters and the Selectable Lasers Module expand the available fluorochrome combinations. So the choice should be addressed by the kind of flow experiments you plan to do. Accuri C6 is a digital PC based bench top analyser, 2 laser system. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for microbiology applications. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. Screw in the other two mounting bolts. No. Researchers studying embryonic stem cells (ESCs) must constantly monitor the pluripotency of their cultures, while those studying human induced pluripotent stem cells (hiPSCs) must assess reprogramming success as well as pluripotency. Click the link below to read about the impactful work done by the small champion, BD Accuri™. Resolution of four microbial populations using light scatter and an autofluorescence trigger, Live/dead discrimination of E. coli using the BD™ Cell Viability Kit. Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. Increasingly, however, research professors and other academic instructors report that they are including active flow cytometry usage in their undergraduate curricula. They are processed directly in the BD Accuri C6 Plus cytometer, saving time. BD Accuri C6 Plus flow cytometer. See also the separate tab for Industrial Applications that use microbes for bioprocessing, food and beverage processing, and biofuels. Free BD Accuri software templates matched to each kit include predefined workspaces, markers, regions, gates, and parameter names for quick and easy setup and analysis. This option significantly expands the fluorochrome combinations you can analyze. Learn more. Intensive testing ensures that the optical and fluidic design can withstand rugged conditions. Not for use in diagnostic or therapeutic procedures. Not for use in diagnostic or therapeutic procedures. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. No. Data was acquired on a BD Accuri C6 and analyzed using BD Accuri C6 software. All reagents and kits are compatible with both BD Accuri™ C6 Plus and Accuri™ C6 flow cytometer systems. All samples were prepared using the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. 340242), acquired, and analyzed on the BD Accuri C6 flow cytometer. Students obtain blood differentials on peripheral blood smears. How you intend to take your Flowcytometry work forward in the years >to come > > >We do not have an Accuri, but an Accuri C6 is a 4 color (2 lasers) >instrument, with some researchers claiming it to be an excellent user >friendly machine, simple to use especially for repetitive experiments, >inexpensive, and easy to train novices on. In the standard configuration of the BD Accuri C6 Plus, three detectors read fluorescence emissions from fluorochromes excited by the blue laser, while the fourth detector reads emissions from fluorochromes excited by the red laser (3-blue/1-red). Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). © 2020 BD. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for industrial applications in bioprocessing, biofuels, agriculture, fermentation, and other enterprises.
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